Chromatography and Spectroscopy Articles

A Clear, Scientist-Focused Explanation of How Large Language Models Process Language, Context, and Prompts

A Practical Guide to Artificial Intelligence and Large Language Models for Chemists, Scientists, and Laboratory Professionals

How Chemistry Educators Can Generate Accurate, Engaging Quizzes in Seconds Using Generative AI

Harnessing Generative AI to Accelerate Chemical Research, Enhance Scientific Learning, and Transform How Chemists Discover, Analyze, and Communicate Science

If you’ve ever wondered how scientists can detect tiny amounts of chemicals in medicine, food, or water, HPLC chromatography is one of the most important tools they use. While the name sounds complex, the basic idea behind HPLC is surprisingly simple—it’s about separating mixtures so we can see what’s inside them.

Liquid chromatography-mass spectrometry (LC-MS) is a vital analytical technique. However, ion suppression is a significant challenge impacting results.
Ion suppression reduces a mass spectrometer's ability to detect target ions. It occurs when co-eluting matrix components interfere with the ionization process in the ion source (e.g., ESI).

Choosing the right C18 column is key to achieving accurate, efficient separations. Factors such as particle size, pore size, and column dimensions affect resolution and run time. This section will help you match column properties to your sample type and analytical goals — whether for pharmaceuticals, environmental samples, or complex mixtures.

High-Performance Liquid Chromatography (HPLC) pumps serve as the critical heartbeat of your analytical system, delivering mobile phase with exceptional precision through the chromatographic column. These sophisticated instruments must maintain constant flow rates—typically ranging from microliters to milliliters per minute—while generating pressures up to 6,000 psi or higher.