UV-Visible Spectroscopy – Resources List
Electronic Transitions in UV-Visible Spectroscopy: pi->pi*, n->pi*, and Charge Transfer Explained
Understand the electronic transitions responsible for UV-Vis absorption and how molecular structure controls spectral features.
Chromophores and Auxochromes in UV-Visible Spectroscopy: Structure-Spectrum Relationships
Learn how chromophores and auxochromes control UV-Vis absorbance, wavelength shifts, and intensity changes.
Molar Absorptivity in UV-Visible Spectroscopy: Meaning, Units, and Practical Use
Discover what molar absorptivity really represents and how to use epsilon values correctly in UV-Vis calculations.
Single-Beam vs Double-Beam UV-Vis Spectrophotometers: Differences, Advantages, and Limitations
Compare single-beam and double-beam UV-Vis instruments and learn which design best fits your analytical needs.
UV-Visible Light Sources Explained: Deuterium and Tungsten Lamps in Spectrophotometry
Learn how UV-Vis lamps work, how they age, and how lamp problems affect spectral quality.
Spectral Bandwidth in UV-Visible Spectroscopy: Why Slit Width Matters
Understand how spectral bandwidth affects resolution, sensitivity, and quantitative accuracy in UV-Vis analysis.
UV-Vis Detectors Explained: Photomultiplier Tubes vs Photodiode Arrays
Compare PMT and photodiode array detectors and learn how detector choice affects speed, noise, and sensitivity.
UV-Vis Cuvette Materials Compared: Quartz vs Glass vs Plastic
Choose the correct cuvette material for UV-Vis analysis and avoid wavelength cutoff and solvent damage issues.
Pathlength Effects in UV-Visible Spectroscopy: Why 1 cm Is Not Always 1 cm
Learn how pathlength variations influence absorbance accuracy in standard and microvolume UV-Vis measurements.
Dirty and Scratched Cuvettes in UV-Vis Spectroscopy: Hidden Sources of Error
See how cuvette contamination and surface damage distort UV-Vis absorbance and baseline stability.
Solvent UV Cutoff Explained: Avoiding Baseline Interference in UV-Vis Spectroscopy
Understand solvent UV cutoff values and prevent baseline noise and absorbance distortion.
Noisy UV-Visible Spectra: Causes, Diagnostics, and Solutions
Identify the root causes of noise in UV-Vis spectra and learn how to restore clean, stable absorbance data.
Baseline Drift in UV-Visible Spectroscopy: Why It Happens and How to Fix It
Learn the common causes of baseline drift in UV-Vis instruments and how to stabilize measurements.
Stray Light in UV-Visible Spectroscopy: Effects on Linearity and Accuracy
Discover how stray light distorts UV-Vis absorbance and reduces Beer-Lambert linearity.
Negative Absorbance in UV-Visible Spectroscopy: Causes and Corrective Actions
Learn why negative absorbance occurs in UV-Vis analysis and how to correct blanking and reference errors.
Peak Shifts in UV-Visible Spectroscopy: Bathochromic and Hypsochromic Effects Explained
Understand why UV-Vis absorption peaks shift with solvent, pH, and chemical interactions.
Wavelength and Photometric Accuracy in UV-Visible Spectroscopy
Learn how wavelength and absorbance accuracy are verified in UV-Vis instruments.
UV-Vis Calibration Curves: Best Practices for Linearity and Quantitation
Build reliable UV-Vis calibration curves and avoid common concentration and range errors.
LOD and LOQ in UV-Visible Spectroscopy: Calculation and Interpretation
Understand how detection and quantitation limits are determined in UV-Vis methods.
Protein and DNA Quantification by UV-Visible Spectroscopy (260/280 Ratios Explained)
Learn how UV-Vis is used for protein and nucleic acid analysis and how to interpret purity ratios.
pH Effects in UV-Visible Spectroscopy: Acid-Base Equilibria and Spectral Changes
Explore how pH alters UV-Vis absorbance through chemical equilibria and structural changes.
UV-Visible Spectroscopy in Environmental Analysis: Nitrates, Metals, and Dyes
See how UV-Vis spectroscopy is applied to environmental and water analysis.
Derivative UV-Visible Spectroscopy: Enhancing Resolution and Selectivity
Learn when and why derivative UV-Vis spectroscopy improves peak separation.
Common UV-Visible Spectroscopy Method Development Mistakes (And How to Avoid Them)
Avoid the most common UV-Vis method development errors that compromise accuracy and reliability.
Beer-Lambert Law in UV-Visible Spectroscopy: Theory, Assumptions, and Common Failures
Learn how the Beer-Lambert law works in UV-Vis spectroscopy, why it fails in real samples, and how to restore linear absorbance behavior.