About
This advanced HPLC and UHPLC course provides in-depth training in chromatographic troubleshooting, system diagnostics, and method optimization for analytical chemists working in pharmaceutical, environmental, clinical, and industrial laboratories. The program focuses on practical problem-solving in high performance liquid chromatography systems, including advanced peak analysis, resolution failures, retention time instability, excessive backpressure, baseline drift, gradient artifacts, and detector-related issues. Participants learn how to diagnose problems systematically using chromatographic theory and instrument-level understanding. Special emphasis is placed on UHPLC-specific considerations such as high-pressure fitting integrity, column particle size effects, extracolumn dispersion, dwell volume impact, and gradient delay volume management. The course explains how system parameters—including mobile phase composition, pH, temperature, flow rate, column chemistry, and injection conditions—interact to influence efficiency, selectivity, and reproducibility. Method optimization modules cover selectivity control, gradient design strategy, peak symmetry improvement, resolution enhancement, and robustness evaluation. Troubleshooting workflows are structured to distinguish between column-related issues, mobile phase problems, hardware malfunctions, and method design limitations. Designed for experienced HPLC users seeking advanced competence, this online training builds the expertise required to restore system performance, improve chromatographic robustness, and confidently solve complex separation challenges.
You can also join this program via the mobile app. Go to the app